Pemberitahuan: Untuk pengalaman terbaik, disarankan menggunakan desktop/laptop untuk mengakses alat ini.
instructions:
- Mouse: Click and drag the tube to move it
- Keyboard: Use arrow keys (↑↓←→) to move the tube precisely
- Zoom: Use +/- buttons to zoom in/out for better precision
- Fullscreen: Click the fullscreen button for maximum viewing area
- Red horizontal lines - 0% reference (RBC/putty boundary)
- Blue horizontal lines - 100% reference (plasma/empty boundary)
- Purple marker - Reading point (RBC/plasma boundary)
100%
Keyboard: Arrow keys to move tube • ESC: Exit fullscreen
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Hematocrit Reading:
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% PCV
Controls
Actual Value:
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Clinical Status:
Difference:
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Proper Microhematocrit Reading Procedure
- Position the tube: Align the boundary between RBCs and putty with the "0" line on the scale
- Adjust the scale: Move the tube so the top of the plasma column (boundary with empty space) aligns with the "100%" line
- Read the value: The hematocrit value is read where the boundary between RBCs and plasma/buffy coat intersects the scale
- Use either side: You can read from either the left side (fewer markings) or right side (more detailed) of the trapezoid scale
- Check your accuracy: Click "Show Actual Value" to verify your reading
The Enhanced Micro Hematocrit Scale Reader Simulator is a comprehensive educational tool designed for training medical laboratory technicians, students, and healthcare professionals in the proper technique of reading hematocrit values using a 75mm microhematocrit reader with a trapezoid scale.
This simulator addresses the critical need for hands-on practice in hematocrit determination - a fundamental laboratory skill used to measure the packed cell volume (PCV) of red blood cells in blood samples. The tool provides a safe, repeatable learning environment where students can master the technique without the need for actual blood samples or expensive laboratory equipment.
Standard Microhematocrit Procedure
Equipment Required:- Microhematocrit tubes (75mm long, 1.2mm inner bore)
- Clay sealing compound
- Microhematocrit centrifuge (10,000-15,000g RCF capability)
- Microhematocrit tube reader (trapezoid scale)
- EDTA anticoagulated whole blood specimen
- Sample Collection: Allow well-mixed anticoagulated whole blood to enter two microhematocrit tubes until they are approximately two-thirds filled with blood. Avoid air bubbles which indicate poor technique.
- Tube Sealing: Seal one end of each microhematocrit tube with clay material by placing the dry end vertically into the clay (90° angle). The plug should be 4-6mm long. Ensure blood is not forced out during sealing.
- Centrifuge Setup: Place the two microhematocrit tubes in radial grooves of the centrifuge head exactly opposite each other, with sealed ends away from the center.
- Centrifugation: Centrifuge for 5 minutes at maximum speed (10,000-15,000g RCF). The centrifuge should reach maximum speed within 30 seconds.
- Reading Setup: Remove tubes immediately after centrifuge stops. Position the tube on the microhematocrit reader with the RBC/putty boundary aligned with the 0% line.
- Scale Alignment: Adjust the tube position so the plasma/air boundary aligns with the 100% line on the trapezoid scale.
- Hematocrit Reading: Read the hematocrit value where the RBC/plasma (buffy coat) boundary intersects the scale. The buffy coat should NOT be included in the reading.
- Quality Control: Duplicate results should agree within 1%. If not, repeat the procedure. Record results and perform clinical interpretation.
Critical Points for Accurate Results:
- Proper sealing: Incomplete sealing causes falsely low results
- Adequate centrifugation: Insufficient time yields falsely elevated values
- Immediate reading: Delays after centrifugation affect accuracy
- Buffy coat exclusion: Do not include white cell layer in measurement
- Parallax avoidance: Read at eye level to prevent optical errors
Key Features
- Realistic Simulation: Accurate representation of 75mm microhematocrit reader with trapezoid scale. Visual simulation includes all blood components: empty space, plasma, buffy coat, RBC layer, and sealing putty.
- Interactive Learning: Drag-and-drop tube positioning, keyboard precision controls, real-time reading display, and dual-scale practice (10% and 5% increments).
- Advanced Controls: 0.5x to 3x zoom functionality, fullscreen mode, reference guides, alignment markers, and keyboard shortcuts for enhanced precision.
- Clinical Scenarios: Practice with normal values, anemia, polycythemia, dehydration, and hemodilution cases. Adjustable parameters and random sample generation.
- Performance Assessment: Accuracy feedback, clinical interpretation, error analysis, and educational alerts for continuous improvement.
- Universal Access: Responsive design for desktop, tablet, and mobile. No installation required - runs in any modern web browser.
Learning Objectives
Upon completion of training with this simulator, users will be able to:
- Properly position a microhematocrit tube on a trapezoid scale reader
- Accurately align the RBC/putty boundary with the 0% line
- Correctly align the plasma/air boundary with the 100% line
- Precisely read hematocrit values from the RBC/plasma boundary intersection
- Differentiate between readings from left and right sides of the trapezoid scale
- Interpret clinical significance of hematocrit values
- Recognize common reading errors and troubleshooting techniques
Normal Reference Ranges
| Population | Hematocrit Range (%) |
|---|---|
| Adult Males | 40-55% |
| Adult Females | 36-48% |
| Newborns | 45-60% |
| 1 Year Old | 27-44% |
| Children (2-18 years) | Gradually increases to adult levels |
About the Trapezoid Scale
The trapezoid-shaped scale has two sides with different markings:
- Left side: Less detailed markings (every 10%)
- Right side: More detailed markings (every 5%)
You can read the hematocrit value from either side of the scale based on where the RBC/plasma boundary intersects the scale. The right side offers more precise readings due to its finer graduations.
Literatur
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