Reticulocyte Counting Simulator - Miller Disc Method

instructions:

1. Examine the field at 1000× magnification (oil immersion)
2. Count reticulocytes (blue cells with dark granules) in the large square
3. Count all RBCs (including reticulocytes) in the small square
4. Apply edge rule: Don't count cells touching bottom/right edges of small square
5. Calculate percentage using the formula: Retics in large square × 100 ÷ (RBCs in small square × 9)
6. Count minimum 2,000 cells for adequate precision (multiple fields)

Cell Identification:

Mature RBC

Reticulocyte (NMB+)

Microscope View - Oil Immersion (1000x)

Normal Range

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Manual Counting

Reticulocytes in Large Square:

RBCs in Small Square:

Remember the edge rule!

Results & Calculation

Retics (%) = - × 100
             (- × 9)
        = -.--%

Click Calculate to see results

Reference Values (NMB Method)

• Normal: 20-110 × 10⁹/L (0.5-2.5%)
• Low: <20 × 10⁹/L (<0.5%)
• High: >110 × 10⁹/L (>3.0%)
• Critical High: >200 × 10⁹/L (>5.0%)